doi: 10.5660/WTS.2020.9.4.319 Citation BibTeX RIS Mendeley

Mechanism of Resistance to ALS Inhibiting Herbicides in Scirpus juncoides Collected in Gyeongnam Province, Korea

경남지방 수집종 올챙이고랭이의 ALS 저해 제초제에 대한 저항성 기작

• Soo Yong Shim
• Sung Hwan Choi
• Jae Ho Kim
• Jung-Sung Chung
• WeiQiang Jia
• Kee Woong Park
• Jeung Joo Lee

This study was conducted to determine the degree of resistance and resistance mechanism to acetolactate synthase (ALS) inhibiting herbicides in Scirpus juncoides biotypes collected in eight cities and counties in Gyeongsangnam-do. Among the S. juncoides biotypes, Goseong28 biotype was highly resistant to penoxsulam and flucetosulfuron. Based on the als gene sequencing, no mutation was found in the Goseong28 biotype. When the ALS activity of Goseong28 biotype was compared with Tongyeong238 biotype which was susceptible to penoxsulam, no significant difference was found. On the other hand, the sensitivity to penoxsulam and flucetosulfuron in the Goseong28 biotype was significantly increased in pretreatment of fenitrothion, a P450 inhibitor. It was considered that the resistance to the ALS-inhibiting herbicide in the Goseong28 biotype was not due to the mutation of als or an increase in enzymatic activity, but due to the non-target site resistance (NTSR) of P450. The low resistance to flucetosulfuron in Haman135 and Tongyeong238 was considered to be due to mutations of Pro197 of the als gene to Ser and Ala. This is the first report related to the NTSR in the S. juncoides to ALS-inhibiting herbicides, and may be used as an important reference material for studies related to the herbicide resistance in S. juncoides.