{"doi":"10.5660/WTS.2020.9.3.219","aliasDoi":null,"conflicted":false,"contentType":"ARTICLE","contentGbNm":"학술 논문","depositorId":"ksws","depositorName":"한국잡초학회,한국잔디학회","submissionId":"2020-0474156","lhsRegState":"1","lhsRegDt":null,"insertDt":"20201007101310","lastUpdateDt":"20201007101310","deleteFlg":"N","deleteType":null,"memo":null,"deleteDt":null,"inProgressOfRemoving":false,"doiLocationList":[{"doi":"10.5660/WTS.2020.9.3.219","seqNo":1,"url":"http://www.weedturf.org/article/?num=N0260090302","insertDt":"20201007101310","lastUpdateDt":"20201007101310"}],"contributorList":[{"doi":"10.5660/WTS.2020.9.3.219","seqNo":1,"conRole":null,"conName":null,"surname":"김","surnameEn":"Kim","givenName":"보관","givenNameEn":"Bo Gwan ","organName":"한국화학연구원 의약바이오연구본부 친환경신물질연구센터","organNameEn":"Research Center for Eco-Friendly New Materials, Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea","emailAddr":null,"publisherId":null,"kistiPsonId":null,"orcid":"https://orcid.org/0000-0002-0031-9033","isni":null,"insertDt":"20201007101310","lastUpdateDt":"20201007101310","representativeName":"김보관","representativeNames":["김","보관"]},{"doi":"10.5660/WTS.2020.9.3.219","seqNo":2,"conRole":null,"conName":null,"surname":"김","surnameEn":"Kim","givenName":"진석","givenNameEn":"Jin-Seog ","organName":"한국화학연구원 의약바이오연구본부 친환경신물질연구센터","organNameEn":"Research Center for Eco-Friendly New Materials, Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea","emailAddr":null,"publisherId":null,"kistiPsonId":null,"orcid":"https://orcid.org/0000-0002-9939-522X","isni":null,"insertDt":"20201007101310","lastUpdateDt":"20201007101310","representativeName":"김진석","representativeNames":["김","진석"]}],"referenceList":[],"changeHistoryList":[],"doiAttachList":[],"relativeLinkList":[],"citingArticleList":[],"contentRemovingList":null,"relativeIdsList":null,"doiLocChangedYn":null,"registStatus":null,"status":null,"messageId":null,"message":null,"title":"개구리밥(Spirodela polyrhiza) turion을 이용한 신규 제초활성물질 검정법의 확립","titleEn":"Establishment of In vivo Bioassay Using Giant Duckweed (Spirodela polyrhiza L.) Turion for an Efficient Screening of New Herbicidal Compounds","pubYear":"2020","pubMonth":"9 ","pubDay":"30","publisherId":null,"lang":null,"pubCountry":null,"conflictId":null,"startPg":"219","endPg":"234","abs":"연구는 개구리밥 튜리온을 이용하여 신규 제초활성물질의 고효율 탐색을 위한in vivo HTS를 확립하기 위해 수행되었다. 스크리닝 방법의 신뢰도를 높이기 위해 최아된 turion을 선별하여 접종하고, 화합물 용해를 위해 0.5% 이하의 DMSO를 사용하는 것이 적합하였다. 본 연구와 이전에 보고된 결과를 바탕으로 turion 준비, 약제조제, 최적 배양조건, 활성검정 기준, 증상분류 기준 등을 확립한 후 48 well plate 또는 96 well plate에서 수행할 수 있는 표준 프로토콜을 확립하여 \"SPIPO turion bioassay\"라고 명명한 기본 검정법을 구축하였다. 이후 서로 다른 작용기작을 가진 26종 대표 제초제들을 대상으로 활성 정도와 제초증상 특성을 파악한 결과, 각각의 작용성을 잘 나타내었다. 궁극적으로 본 검정방법은 제반특성상(사용되는 turion의유전적균일성, 제초제작용유형구별가능성, 실험조건의확장성, 데이터획득과관리의 다양성, 비교적 간편한 실험방법과 저비용 소요 등) 신규 제초활성물질의in vivo 고효율 검정에 매우 유용하게 활용될 수있을것으로판단되었다.","absEn":"This study was conducted to develop in vivo high-throughput screening (HTS) system using giant duckweed (Spirodela polyrhiza L [SPIPO]) turion for new herbicidal agents. In order to increase the reliability of the screening method, it was appropriate to select and inoculate the best turion, and to use dimethyl sulfoxide of 0.5% or less for dissolution of the compound. Based on the results of this study and previous research, basic screening formats using 48 and 96 well plates were established after settling of preparation of turion and chemical solutions, optimal culture conditions, and evaluating criteria of herbicidal activity and symptoms. Then, 26 representative herbicidal agents with different mechanisms of action were tested for a verification of this system. Finally, in vivo HTS for new herbicidal agents named “SPIPO turion bioassay” and its standard protocol was established. This screening method had several advantages such as a use of turions with genetic uniformity, rough distinction of an action type of screening substances, diversification of the experimental conditions, an acquisition and management of the data for various purposes, relatively simple methods and low costs of experiment tools, etc. and seemed to be very useful for a rapid screening of herbicidal substance with new action site, especially","kwd":null,"vol":"9","issue":"3","ctrlNo":"JRL2017-3044","journalTitle":"Weed & Turfgrass Science","projectId":null,"articleProjectList":[],"pubmedId":null,"journal":{"doi":"10.566","aliasDoi":null,"conflicted":false,"contentType":"JOURNAL","contentGbNm":"저널","depositorId":"ksws","depositorName":"한국잡초학회,한국잔디학회","submissionId":null,"lhsRegState":null,"lhsRegDt":null,"insertDt":"20170630171830","lastUpdateDt":"20170630171830","deleteFlg":"N","deleteType":null,"memo":null,"deleteDt":null,"inProgressOfRemoving":false,"doiLocationList":null,"contributorList":null,"referenceList":null,"changeHistoryList":null,"doiAttachList":null,"relativeLinkList":null,"citingArticleList":null,"contentRemovingList":null,"relativeIdsList":null,"doiLocChangedYn":null,"registStatus":null,"status":null,"messageId":null,"message":null,"title":"Weed & Turfgrass Science","titleEn":"Weed & Turfgrass Science","pubYear":"2012","pubMonth":null,"pubDay":null,"publisherId":"JDHHCQ","lang":"KO","pubCountry":"KR","conflictId":null,"ctrlNo":"JRL2017-3044","titleAbbr":null,"issnP":"2287-7924","issnE":"2288-3312","issnL":null,"pubName":"한국잡초학회,한국잔디학회","pubTerm":"M3-01","pubTermText":null,"url":"http://www.weedturf.org","preCtrlNo":null,"preTitle":"","volumeIssueCount":56,"articleCount":571,"articleList":null,"volumeIssueList":null,"issn":"2287-7924","conflictDoiList":null,"secondDoiLocation":null,"contentGb":"01","firstDoiLocation":null},"conflictDoiList":null,"secondDoiLocation":null,"contentGb":"03","firstDoiLocation":{"doi":"10.5660/WTS.2020.9.3.219","seqNo":1,"url":"http://www.weedturf.org/article/?num=N0260090302","insertDt":"20201007101310","lastUpdateDt":"20201007101310"}}