doi: 10.7744/KJOAS.20180044 BibTeX RIS Mendeley


NMR-based metabolomic profiling of the liver, serum, and urine of piglets treated with deoxynivalenol

  • Jin Young Jeong (Animal Nutrition & Physiology Team, National Institute of Animal Science)
  • Min Seok Kim (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University)
  • Hyun Jung Jung (Animal Nutrition & Physiology Team, National Institute of Animal Science)
  • Min Ji Kim (Animal Nutrition & Physiology Team, National Institute of Animal Science)
  • Hyun Jeong Lee (Animal Nutrition & Physiology Team, National Institute of Animal Science)
  • Sung Dae Lee (Animal Nutrition & Physiology Team, National Institute of Animal Science)

초록

Deoxynivalenol (DON), a Fusarium mycotoxin, causes health hazards for both humans and livestock. Therefore, the aim of this study was to investigate the metabolic profiles of the liver, serum, and urine of piglets fed DON using proton nuclear magnetic resonance ($^1H-NMR$) spectroscopy. The $^1H-NMR$ spectra of the liver, serum, and urine samples of the piglets provided with feed containing 8 mg DON/kg for 4 weeks were aligned and identified using the icoshift algorithm of MATLAB $R^2013b$. The data were analyzed by multivariate analysis and by MetaboAnalyst 4.0. The DON-treated groups exhibited discriminating metabolites in the three different sample types. Metabolic profiling by $^1H-NMR$ spectroscopy revealed potential metabolites including lactate, glucose, taurine, alanine, glycine, glutamate, creatine, and glutamine upon mycotoxin exposure (variable importance in the projection, VIP > 1). Forty-six metabolites selected from the principal component analysis (PCA) helped to predict sixty-five pathways in the DON-treated piglets using metabolite sets containing at least two compounds. The DON treatment catalyzed the citrate synthase reactions which led to an increase in the acetate and a decrease in the glucose concentrations. Therefore, our findings suggest that glyceraldehyde-3-phosphate dehydrogenase, citrate synthase, ATP synthase, and pyruvate carboxylase should be considered important in piglets fed DON contaminated feed. Metabolomics analysis could be a powerful method for the discovery of novel indicators underlying mycotoxin treatments.


발행 정보.

서지정보
Korean Journal of Agricultural Science / v.45, no.3, 2018, pp.455-461
발행기관
Institute of Agricultural Science, Chungnam National University
저널DOI
doi: 10.7744
pISSN
2466-2402
eISSN
2466-2410
발행일
2018.9 .30
언어
한국어

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피인용 문헌

  1. "Genome-Wide Transcriptome and Metabolome Analyses Provide Novel Insights and Suggest a Sex-Specific Response to Heat Stress in Pigs" Genes, vol. 11(5), p.540-. 2020. doi:10.3390/genes11050540
  2. "Saccharomyces cerevisiae partially to completely ameliorates the adverse effects of aflatoxin on the in vitro rumen fermentation of buffalo diet" Korean journal of agricultural science, vol. 48(1), p.73-. 2021. doi:10.7744/kjoas.20210001

이력 정보

2018.04.10 투고
2018.06.08 수정
2018.06.14 승인
2018.09.30 발행

관련 과제

  • National Institute of Animal Sciences 후원

위치 정보


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