doi: 10.5423/PPJ.OA.10.2019.0268 BibTeX RIS Mendeley


Wisteria Vein Mosaic Virus Detected for the First Time in Iran from an Unknown Host by Analysis of Aphid Vectors

Wisteria Vein Mosaic Virus Detected for the First Time in Iran from an Unknown Host by Analysis of Aphid Vectors

  • Valouzi,Hajar(Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University) ,
  • Hashemi,Seyedeh-Shahrzad(Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University) ,
  • Wylie,Stephen J.(Plant Biotechnology Research Group - Virology, State Agricultural Biotechnology Centre, Murdoch University) ,
  • Ahadiyat,Ali(Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University) ,
  • Golnaraghi,Alireza(Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University) ,

초록

The development of reverse transcription-polymerase chain reaction using degenerate primers against conserved regions of most potyviral genomes enabled sampling of the potyvirome. However, these assays usually involve sampling potential host plants, but identifying infected plants when they are asymptomatic is challenging, and many plants, especially wild ones, contain inhibitors to DNA amplification. We used an alternative approach which utilized aphid vectors and indicator plants to identify potyviruses capable of infecting common bean (Phaseolus vulgaris). Aphids were collected from a range of asymptomatic leguminous weeds and trees in Iran, and transferred to bean seedlings under controlled conditions. Bean plants were tested serologically for potyvirus infections four-weeks postinoculation. The serological assay and symptomatology together indicated the presence of one potyvirus, and symptomology alone implied the presence of an unidentified virus. The partial genome of the potyvirus, encompassing the complete coat protein gene, was amplified using generic potyvirus primers. Sequence analysis of the amplicon confirmed the presence of an isolate of Wisteria vein mosaic virus (WVMV), a virus species not previously identified from Western Asia. Phylogenetic analyses of available WVMV sequences categorized them into five groups: East Asian-1 to 3, North American and World. The Iranian isolate clustered with those in the World group. Multiple sequence alignment indicated the presence of some genogroup-specific amino acid substitutions among the isolates studied. Chinese isolates were sister groups of other isolates and showed higher nucleotide distances as compared with the others, suggesting a possible Eastern-Asian origin of WVMV, the main region where Wisteria might have originated.


키워드

aphid-transmission approaches;potyvirome;potyviruses;vectorome;Wisteria vein mosaic virus;


발행 정보

서지정보
The Plant Pathology JournalThe Plant Pathology Journal / v.36, no.1, 2020년, pp.87-97
발행기관
한국식물병리학회
저널 DOI
doi: 10.5423
pISSN
1598-2254
eISSN
2093-9280
발행일
2020.02.01
언어
영어

이력 정보

2020.02.01 발행
2020.02.10 DOI발급

위치 정보


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