doi: 10.5423/PPJ.OA.08.2019.0227 BibTeX RIS Mendeley


Ralstonia solanacearum Type III Effectors with Predicted Nuclear Localization Signal Localize to Various Cell Compartments and Modulate Immune Responses in Nicotiana spp.

Ralstonia solanacearum Type III Effectors with Predicted Nuclear Localization Signal Localize to Various Cell Compartments and Modulate Immune Responses in Nicotiana spp.

  • Jeon,Hyelim(Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University) ,
  • Kim,Wanhui(Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University) ,
  • Kim,Boyoung(Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University) ,
  • Lee,Sookyeong(Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University) ,
  • Jayaraman,Jay(Department of Life Sciences, Pohang University of Science and Technology) ,
  • Jung,Gayoung(Department of Life Sciences, Pohang University of Science and Technology) ,
  • Choi,Sera(Department of Life Sciences, Pohang University of Science and Technology) ,
  • Sohn,Kee Hoon(Department of Life Sciences, Pohang University of Science and Technology) ,
  • Segonzac,Cecile(Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University) ,

초록

Ralstonia solanacearum (Rso) is a causal agent of bacterial wilt in Solanaceae crops worldwide including Republic of Korea. Rso virulence predominantly relies on type III secreted effectors (T3Es). However, only a handful of Rso T3Es have been characterized. In this study, we investigated subcellular localization of and manipulation of plant immunity by 8 Rso T3Es predicted to harbor a nuclear localization signal (NLS). While 2 of these T3Es elicited cell death in both Nicotiana benthamiana and N. tabacum, only one was dependent on suppressor of G2 allele of skp1 (SGT1), a molecular chaperone of nucleotide-binding and leucine-rich repeat immune receptors. We also identified T3Es that differentially regulate flg22-induced reactive oxygen species production and gene expression. Interestingly, several of the NLS-containing T3Es translationally fused with yellow fluorescent protein accumulated in subcellular compartments other than the cell nucleus. Our findings bring new clues to decipher Rso T3E function in planta.


키워드

bacterial wilt;innate immunity;Nicotiana spp.;Ralstonia solanacearum;type III effectors;


발행 정보

서지정보
The Plant Pathology JournalThe Plant Pathology Journal / v.36, no.1, 2020년, pp.43-53
발행기관
한국식물병리학회
저널 DOI
doi: 10.5423
pISSN
1598-2254
eISSN
2093-9280
발행일
2020.02.01
언어
영어

이력 정보

2020.02.01 발행
2020.02.10 DOI발급

위치 정보


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