doi: 10.4489/KJM.520416 Citation BibTeX RIS Mendeley

Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards

Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards

• Ju-Heon Lee (Apple Research Center, National Institute of Horticultural & Herbal Science, Gunwi 43100, Korea)
• Youngsoo Kim (Apple Research Center, National Institute of Horticultural & Herbal Science, Gunwi 43100, Korea)
• Jong-Taek Park (Apple Research Center, National Institute of Horticultural & Herbal Science, Gunwi 43100, Korea)
• Dong-Hyuk Lee (Apple Research Center, National Institute of Horticultural & Herbal Science, Gunwi 43100, Korea)
• Hee-Young Jung (Department of Plant Medicine, Kyungpook National University, Daegu 41566, Korea)

A fungus was isolated from ambrosia beetles collected using beetle traps in an apple orchard in Gunwi-gun, Daegu, South Korea. This fungal strain was termed ARI-24-A5, and was identified through OPEN ACCESS pISSN : 0253-651X eISSN : 2383-5249 Kor. J. Mycol. 2024 December, 52(4):381-389 https://doi.org/10.4489/kjm.520416 Received: December 16, 2024 Revised: December 20, 2024 Accepted: December 20, 2024 © 2024 THE KOREAN SOCIETY OF MYCOLOGY. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http: //creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. morphological characterization and molecular phylogenetic analysis. After 8 d of incubation on potato dextrose agar (PDA), ARI-24-A5 exhibited gray-to-olive coloration, abundant aerial mycelia, and a colony diameter of 72.0–79.0 mm. Morphologically, the aleurioconidiophores formed monilioid chain structures, and the size of the aleurioconidia was 11.1 × 10.8 μm. For precise identification, molecular phylogenetic analysis was performed using the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF1-α), small subunit of nuclear ribosomal RNA (SSU), and RNA polymerase II subunit 1 (RPB1) gene sequences. The overall analysis confirmed that ARI-24-A5 belongs to the genus Ambrosiella, which is known for its symbiotic relationship with ambrosia beetles. In the phylogenetic tree, ARI-24-A5 shared the same taxonomic position as A. catenulata and its morphological characteristics were consistent with those of this species. Therefore, ARI-24-A5 was identified as A. catenulata, making this the first record of this species in South Korea.