doi: 10.4489/KJM.520107 Citation BibTeX RIS Mendeley

Correlation Analysis Study Between Spent Mushroom Substrate and Microbial Community

Correlation Analysis Study Between Spent Mushroom Substrate and Microbial Community

• In Kyu Lee (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Korea)
• Hyun Seung Kim (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Korea)
• Ji Min Woo (Department of Applied Plant Sciences, Kangwon National University, Chuncheon 24341, Korea)
• Won Jun Chang (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Korea)
• Eun Jeong Byeon (Department of Applied Plant Sciences, Kangwon National University, Chuncheon 24341, Korea)
• Ki Byung Park (Jungbubiotech, Wonju 26356, Korea)
• Youn Su Lee (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Korea)

Next-generation sequencing of shiitake, oyster, and king oyster spent mushroom substrates collected from Chun-cheon, Yeo-ju, Hong-cheon, Gwang-ju, Ui-ryeong, and A-san was performed. Metabarcoding analysis using amplicon sequence variants was performed to confirm the microbial content ratio in the medium after harvesting the collected mushrooms; the ratio of the contents of various microorganisms in the medium after mushroom harvest varied depending on the materials added to produce the mushroom medium. The WPGMA analysis of the similarity between microbial communities, which was based on the β-diversity, confirmed that the microbial communities in the substrates of the different mushroom varieties were similar.