doi: 10.4489/KJM.20230039 Citation BibTeX RIS Mendeley

Characteristics of Lentinula edodes Fruiting Bodies Formed by Mono-mono Hybridization Using Mating Type Analysis

Characteristics of Lentinula edodes Fruiting Bodies Formed by Mono-mono Hybridization Using Mating Type Analysis

• Yeongseon Jang (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon 16631, Korea)
• Mi-Jeong Park (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon 16631, Korea)
• Eunjin Kim (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon 16631, Korea)
• Min-Jun Kim (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon 16631, Korea)
• Yeun Sug Jeong (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon 16631, Korea)
• Kang-Hyeon Ka (Forest Microbiology Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon 16631, Korea)

In this study, dikaryotic strains of Lentinula edodes were generated by mono-mono hybridization using mating type analysis and their fruiting body characteristics were investigated. Approximately 100 monokaryotic strains were isolated from the basidiospores of Sanbaekhyhang, and homokaryotic strains were isolated from Chungheung 1ho using protoplast isolation and regeneration. Their mating types were evaluated and a total of 60 dikaryotic strains were hybridized. Using these strains, fruiting bodies were produced and their characteristics were examined after cultivation on sawdust media. The results indicated that the rate of hybridization was 100% and that 55 of 60 strains formed fruiting bodies. These showed normal pileus and gill structures; however,10 strains also produced fruiting bodies with abnormal pileus and gill structures. The weight and size of the fruiting bodies differed depending on the strains. Overall, further studies are needed for predicting the characteristics of hybridized strains based on their parental strains.