doi: 10.4489/KJM.2016.44.4.289 BibTeX RIS Mendeley

Genotyping of the Wild Mushroom Lentinula edodes from Mt. Jungwang and Mt. Gariwang in Korea

  • Jeong, Yeun Sug (Division of Wood Chemistry and Microbiology, National Institute of Forest Science)
  • Jang, Yeongseon (Division of Wood Chemistry and Microbiology, National Institute of Forest Science)
  • Ryoo, Rhim (Division of Wood Chemistry & Microbiology, National Institute of Forest Science)
  • Kim, Ki-Hwan (C&K Genomics)
  • Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, National Institute of Forest Science)

Abstract

Lentinula edodes is an edible wild mushroom that can be found in mountainous regions of the Korean peninsula. Wild oak mushrooms were collected from Quercus mongolica at an elevation of more than 1,000 m on Mt. Jungwang and Mt. Gariwang in Gangwon province. We examined 10 oak mushroom strains to evaluate the genetic similarity among strains. Genetic similarity was determined based on the analysis of microsatellite markers (Led A2, Led A8, Led B2, Led B6, and Led D6) registered in the National Center for Biotechnology Information. We also performed dual culture tests on potato dextrose agar for 2 months at $25^{\circ}C$. The observed heterozygosity across all microsatellites ranged from 0.00 and 0.60 among 5 microsatellite markers, and the polymorphism information content values of Led A2, Led A8, Led B2, Led B6, and Led D6 were 0.0000, 0.8144, 0.6194, 0.4892, and 0.5702, respectively (mean value = 0.4987). Confrontation lines between strains were formed for almost all combinations. In conclusion, the oak mushroom populations of Mt. Jungwang and Mt. Gariwang have mixed gene pools. However, further studies are needed to identify genetic similarities and variations among these populations.

Publication Info.

Bibliography
The Korean Journal of Mycology / v.44, no.4, 2016, pp.289-295
Publisher
한국균학회
Journal DOI
doi: 10.4489
pISSN
0253-651X
eISSN
2383-5249
Date
2016.12.30
Language
Korean

Reference

  1. Kim KH, Kim YY, Ka KH, Lee HS, Bak WC, Jeong SJ, Seong JY, Suh DS, "Microsatellite markers for population-genetic studies of shiitake (Lentinula edodes) strains" Genes Genomics, vol. 31 pp. 421-430. 2009. doi:10.1007/BF03191855
  2. Xiao Y, Liu W, Dai Y, Fu C, Bian Y, "Using SSR markers to evaluate the genetic diversity of Lentinula edodes natural germplasm in China" World J Microbiol Biotechnol, vol. 26 pp. 527-536. 2010. doi:10.1007/s11274-009-0202-4
  3. Liu J, Wang ZR, Li C, Bian YB, Xiao Y, "Evaluating genetic diversity and constructing core collections of Chinese Lentinula edodes cultivars using ISSR and SRAP markers" J Basic Microbiol, vol. 55 pp. 749-760. 2015. doi:10.1002/jobm.201400774
  4. Qin LH, Tan Q, Chen MJ, Pan YJ, "Use of intersimple sequence repeats markers to develop strain-specific SCAR markers for Lentinula edodes" FEMS Microbiol Lett, vol. 257 pp. 112-116. 2006. doi:10.1111/j.1574-6968.2006.00145.x
  5. Karaoglu H, Lee CM, Meyer W, "Survey of simple sequence repeats in completed fungal genomes" Mol Biol Evol, vol. 22 pp. 639-649. 2005. doi:10.1093/molbev/msi057
  6. Terashima K, Matsumoto T, Hasebe K, Fukumasa-Nakai Y, "Genetic diversity and strain-typing in cultivated strains of Lentinula edodes (the shiitake mushroom) in Japan by AFLP analysis" Mycol Res, vol. 106 pp. 34-39. 2002. doi:10.1017/S0953756201005007
  7. Terashima K, Matsumoto T, "Strain typing of shiitake (Lentinula edodes) cultivars by AFLP analysis, focusing on a heatdried fruiting body" Mycoscience, vol. 45 pp. 79-82. 2004. doi:10.1007/S10267-003-0152-X
  8. Liu J, "Power Marker v3.0 manual [Internet]" 2016.
  9. Liu K, Muse SV, "PowerMarker: integrated analysis environment for genetic marker data" Bioinformatics, vol. 21 pp. 2128-2129. 2005. doi:10.1093/bioinformatics/bti282
  10. Nei M, "Estimation of average heterozygosity and genetic distance from a small number of individuals" Genetics, vol. 89 pp. 583-590. 1978.
  11. Xiang X, Li C, Li L, Bian Y, Kwan HS, Nong W, Cheung MK, Xiao Y, "Genetic diversity and population structure of Chinese Lentinula edodes revealed by InDel and SSR markers" Mycol Prog, vol. 15 pp. 37. 2016. doi:10.1007/s11557-016-1183-y
  12. Chiu SW, Wang ZM, Chiu WT, Lin FC, Moore D, "An integrated study of individualism in Lentinula edodes in nature and its implication for cultivation strategy" Mycol Res, vol. 103 pp. 651-660. 1999. doi:10.1017/S095375629900859X
  13. Li AZ, Xu XF, Lin FX, Cheng SM, Lin FC, "Cloning and identification of partial DNA fragment for the B mating-type factor in Lentinula edodes using degenerate PCR" World J Microbiol Biotechnol, vol. 23 pp. 411-415. 2007. doi:10.1007/s11274-006-9239-9
  14. Rice WR, "Analyzing tables of statistical tests" Evolution, vol. 43 pp. 223-225. 1989. doi:10.2307/2409177

History

2016.09.17 SUBMITTED
2016.11.07 REVISED
2016.11.08 ACCEPTED
2016.12.30 PUBLISHED

Project

  • Breeding of new strains of shiitake for cultivar protection and substitution of import / National Institute of Forest Science 후원

Link

Other Types.

닫기