doi: 10.4489/KJM.2015.43.1.71 BibTeX RIS Mendeley

Gibberellins Production and Identification of Endophytic Fungi Isolated from Aquatic Plant in Fresh Water

담수에 자생하는 수생식물에서 분리된 내생균류의 지베렐린 생산과 동정

  • Rhu, Young Hyun (경북농업기술원 유기농업연구소)
  • Kang, Sang-Mo (경북대학교 응용생명과학부)
  • Choi, Yu Mi (농촌진흥청 국립농업과학원 농업유전자원센터)
  • Lee, Myung Chul (농촌진흥청 국립농업과학원 농업유전자원센터)
  • Kim, Jong Guk (경북대학교 생명과학부)

Abstract

Aquatic plant Hydrocharis dubia (Blume) Backer was collected from the Dalsung wetland in Daegu. Sixteen endophytic fungi with different colony morphologies were isolated from the roots of aquatic plants. Waito-c rice (WR) seedlings were treated with fungal culture filtrates (FCF) for screening plant growth-promoting activity. In the results, HD1008 strain isolated from aquatic plant showed highest plant growth-promoting activity. The FCF of HD1008 strain was analyzed using gas chromatography mass spectrometry (GC/MS) with selected ion monitoring (SIM). Analysis of the FCF of HD1008 strain found that it contained gibberellins (GA) ($GA_1$, 1.2 ng/100 mL; $GA_4$, 5 ng/100 mL). Phylogenetic tree of HD1008 strain was constructed by partial internal transcribed spacer (ITS) region and partial beta-tubulin gene sequences. Therefore, we describe HD1008 strain as a new gibberellin-producing Penicillium trzebinskii based on morphological and molecular characteristics.

Publication Info.

Bibliography
The Korean Journal of Mycology / v.43, no.1, 2015, pp.71-76
Publisher
한국균학회
Journal DOI
doi: 10.4489
pISSN
0253-651X
eISSN
2383-5249
Date
2015.03.31
Language
Korean

Reference

  1. Whitaker V, Matvienko B, "The denitrification potential and hydrological conditions in the wetlands of the Lobo resevoir" Proceeding of Verhandlungen Internationale Vereinigung fur Theoretische und Angewandte Limnologie, pp. 1377-1382. 1995.
  2. Denny P, "Biodiversity and wetlands" Wetlands Ecol Manag, vol. 3 pp. 55-61. 1994.
  3. Kuczynska-Kippen N, "Habitat choice in rotifer communities of three shallow lakes: impact of macrophyte substratum and season" Hydrobiologia, vol. 593 pp. 27-37. 2007. doi:10.1007/s10750-007-9073-6
  4. Mitsch WJ, Grosselink JG, "Wetlands" 2000.
  5. Carpenter S, Lodge DM, "Effects of submersed macrophytes on ecosystem processes" Aquatic Bot, vol. 26 pp. 341-370. 1986. doi:10.1016/0304-3770(86)90031-8
  6. Clark SJ, Wharton G, "Sediment nutrient characteristics and aquatic macrophytes in lowland English rivers" Sci Total Environ, vol. 266 pp. 103-112. 2001. doi:10.1016/S0048-9697(00)00754-3
  7. Sand-Jensen K, Pedersen O, "Velocity gradients and turbulence around macrophyte stands in streams" Freshw Biol, vol. 42 pp. 315-328. 1999. doi:10.1046/j.1365-2427.1999.444495.x
  8. Desmet NJ, Van Belleghem S, Seuntjens P, Bouma, TJ, Buis K, Meire P, "Quantification of the impact of macrophytes on oxygen dynamics and nitrogen retention in a vegetated lowland river" Phys Chem Earth, vol. 36 pp. 479-489. 2011. doi:10.1016/j.pce.2008.06.002
  9. Yeh TY, Ke TY, Lin YL, "Algal growth control within natural water purification systems: macrophyte light shading effects" Water Air Soil Pollut, vol. 214 pp. 575-586. 2011. doi:10.1007/s11270-010-0447-4
  10. Kim HJ, Park JS, Kim SY, Koo JG, Bang IC, Kwon SR, "Identification of water mold from wild brook lamprey, Lethenterone reissneri" J Fish Pathol, vol. 26 pp. 39-44. 2013. doi:10.7847/jfp.2012.26.1.039
  11. Jee BY, Lee DC, Kim NY, Jung SH, Park SI, "Identification and chemotherapeutic effects of the fungi from three salmonid species and their eggs" J Fish Pathol, vol. 20 pp. 147-160. 2007.
  12. You YH, Yoon H, Kang SM, Shin JH, Choo YS, Lee IJ, Lee JM, Kim JG, "Fungal diversity and plant growth promotion of endophytic fungi from six halophytes in Suncheon bay" J Microbiol Biotechnol, vol. 22 pp. 1549-1556. 2012. doi:10.4014/jmb.1205.05010
  13. Khan SA, Hamayun M, Yoon H, Kim HY, Suh SJ, Hwang SK, Kim JM, Lee IJ, Choo YS, Yoon UH, et al, "Plant growth promotion and Penicillium citrinum" BMC Microbiol, vol. 8 pp. 231-240. 2008. doi:10.1186/1471-2180-8-231
  14. You YH, Yoon H, Kang SM, Woo JR, Choo YS, Lee IJ, Shin JH, Kim JG, "Cadophora malorum Cs-8-1 as a new fungal strain producing gibberellins isolated from Calystegia soldanella" J Basic Microbiol, vol. 53 pp. 630-634. 2013. doi:10.1002/jobm.201200002
  15. Glass, NL, Donaldson GC, "Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes" Appl Environ Microbiol, vol. 61 pp. 1323-1330. 1995.
  16. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S, "MEGA6: molecular evolutionary genetics analysis version 6.0" Mol Biol Evol, vol. 30 pp. 2725-2729. 2013. doi:10.1093/molbev/mst197
  17. Houbraken J, Samson RA, "Phylogeny of Penicillium and the segregation of Trichocomaceae into three families" Stud Mycol, vol. 70 pp. 1-51. 2011. doi:10.3114/sim.2011.70.01
  18. Houbraken J, Visagie CM, Meijer M, Frisvad JC, Busby PE, Pitt JI, Seifert KA, Louis-Seize G, Demirel R, Yilmaz N, et al, "A taxonomic and phylogenetic revision of Penicillium section Aspergilloides" Stud Mycol, vol. 78 pp. 373-451. 2014. doi:10.1016/j.simyco.2014.09.002
  19. Rodriguez RJ, Henson J, Van Volkenburgh E, Hoy M, Wright L, Beckwith F, Kim YO, Redman RS, "Stress tolerance in plants via habitat-adapted symbiosis" ISME J, vol. 2 pp. 404-416. 2008. doi:10.1038/ismej.2007.106
  20. Redman RS, Sheehan KB, Stout RG, Rodriguez RJ, Henson JM, "Thermotolerance generated by plant/fungal symbiosis" Science, vol. 298 pp. 1581. 2002. doi:10.1126/science.1072191
  21. You YH, Yoon HJ, Woo JR, Seo YG, Shin JH, Choo YS, Lee IJ, Kim JG, "Plant growth promotion activity of endophytic fungi isolated from the roots of Calystegia soldanella" Korean J Microbiol Biotechnol, vol. 39 pp. 324-329. 2011.
  22. Hamayun M, Khan SA, Khan AL, Rehman G, Kim YH, Iqbal I, Hussain J, Sohn EY, Lee IJ, "Gibberellin production and plant growth promotion from pure cultures of Cladosporium sp. MH-6 isolated from cucumber (Cucumis sativus L.)" Mycologia, vol. 102 pp. 989-995. 2010. doi:10.3852/09-261

Cited by

  1. "주남저수지와 동판저수지의 수생식물에서 분리된 내생균류의 비교 분석" 한국균학회지, vol. 43(2), p.92-. 2015. doi:10.4489/KJM.2015.43.2.92

History

2015.01.24 SUBMITTED
2015.02.02 REVISED
2015.02.03 ACCEPTED
2015.03.31 PUBLISHED

Project

  • National Academy of Agricultural Science 후원

Link

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